JJAP Conference Proceedings

JJAP Conf. Proc. 1, 011004 (2013) doi:10.7567/JJAPCP.1.011004

Tempo-Spatial Change of Cellular Stiffness and Geometry in the Process of Developing Epithelial Cell–Cell Adhesion Measured by Atomic Force Microscopy

Ryosuke Tanaka, Takeomi Mizutani, Hisashi Haga, Kazushige Kawabata

  1. Faculty of Advanced Life Sciences, Hokkaido University, Sapporo 060-0810, Japan
  • Received January 15, 2013
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Abstract

For sheet-like tissue, mechanical strength at cell–cell adhesion is important. However, it is unclear how long the mechanical strength is constructed. To clarify temporal change of the strength, we measured elasticity of epithelial cells during cellular migration to cover a vacancy by atomic force microscopy. Immediately after the construction of cell–cell contact, elasticity was low. However, elasticity increased following $90$ min. Time-lapse observation of cellular morphology revealed that cells became spindle-shaped after the construction of cell–cell contact and then became round. The changes in elasticity and morphology would be multi-cellular dynamics in the epithelial wound healing process.

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