JJAP Conf. Proc. 4, 011404 (2016) doi:10.7567/JJAPCP.4.011404
A search for effective microorganisms in garbage treatment
- Department of Applied Chemistry and Biochemical Engineering, Shizuoka University, Hamamatsu 432-8561, Japan
- Received October 09, 2015
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This study intends to develop a new method to detect the microorganisms which exist and function actively in the garbage treatment systems, because truly effective microorganisms have not yet been identified although it is undoubtedly certain that microbial activity is the main cause of the decomposition of organic materials in garbage treatment systems. Direct observation of all living bacteria using fluorescence methods was attempted as a first step in order to investigate whether or not non-culturable bacteria really exist and play a role in the process. It was apparent that direct observation of living bacteria on real samples, such as the residue of a garbage treatment system, presented some difficulties in using fluorescence observation methods, probably due to the existence of water soluble and/or suspended solid constituents, although good results could be obtained using clear samples without impurities. Methanol-sonication treatment was not an effective way to avoid the difficulties.
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- 1 S. Matsuda, R. Iwata, and Y. Uhara, J. Adv. Res. Phys. 3, 011208 (2012).
- 2 G. Muyzer, E. C. de Waal, and A. G. Uitterlinden, Appl. Environ. Microbiol. 59, 695 (1993).
- 3 H. Takaku, S. Kodaira, A. Kimoto, M. Nashimoto, and M. Takagi, J. Biosci. Bioeng. 101, 42 (2006).
- 4 H.-S. Xu, N. Roberts, F. L. Singleton, R. W. Attwell, D. J. Grimes, and R. R. Colwell, Microb. Ecol. 8, 313 (1982).
- 5 R. R. Colwell, P. R. Brayton, D. J. Grimes, D. B. Roszak, S. A. Huq, and L. M. Palmer, Nat. Biotechnol. 3, 817 (1985).
- 6 V. Torsvik, J. Goksoyr, and F. Lisedaae, Appl. Environ. Microbiol. 56, 782 (1990).
- 7 J. Gans, M. Wolonsky, and J. Dunbar, Science 309, 1387 (2005).
- 8 M. Lunau, A. Lemke, and K. Walther, Environ. Microbiol. 7, 961 (2005).
- 9 R. T. Noble and J. A. Fuhrman, Aquat. Microb. Ecol. 14, 113 (1998).
- 10 Y. Bettarel, S.-N. Telesphore, C. Amblard, and H. Laveran, Environ. Microbiol. 66, 2283 (2000).
- 11 N. Yamaguchi, T. Kenzaka, and M. Nasu, Microbes Environ. 12, 1 (1997).
- 12 G. G. Rodriguez et al., Environ. Microbiol. 58, 1801 (1992).
- 13 S. Ullrich et al., Environ. Microbiol. 62, 4587 (1996).